Innovative assets
- 1 assay detergent (french patent method for qualification of detergent by mass spectrometry and its applications No. 2,987,894, 09/13/13)
- 1 item in preparation of this assay, another on a membrane protein structure
- 1 article: Stoichiometry of the MexA-OprM binding, as Investigated by blue native gel electrophoresis. Ferrandez Y Monlezun L, Phan G, H Benabdelhak, Benas P, N Ulryck, Falson P, Ducruix A, Picard M, Broutin I. Electrophoresis. 2012 Apr, 33 (8) : 1282-7
Highlights
1 startup créee pendant le projet (CALIXAR). 10 emplois crées.
1 brevet de dosage des détergents n°2987894, 13/9/13
publications:
Stubborn contaminants: influence of detergents on the purity of the multidrug ABC transporter BmrA. Wiseman B, Kilburg A., Chaptal V., Reyes-Mejia G., C., Sarwan J. Falson P.*, Jault J-M*. PLoS One. 2014 9(12):e114864. doi: 10.1371.
Understanding polyspecificity within the substrate-binding cavity of the human multidrug resistance p-glycoprotein. Martinez L, Arnaud O, Henin E, Chaptal V, Doshi R, Zhang Q, Chang G, Falson P. FEBS J. 2014 281 673-82
Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Ward AB, Szewczyk P, Grimard V, Lee CW, Martinez L, Doshi R, Cregger C, Swartz DJ, Falson PG, Urbatsch IL, Govaerts C, Steyaert J, Chang G. PNAS. 2013 110(33) 13386-91.
Actual results
Innovative Assets
Detail: - 1 assay detergent (french patent method for qualification of detergent by mass spectrometry and its applications No. 2,987,894, 09/13/13) - 1 item in preparation of this assay, another on a membrane protein structure - 1 article Stoichiometry of the
Highlights
Detail: 1 startup cré ee pendant le projet (CALIXAR). 10 emplois cré es. 1 brevet de dosage des dé tergents n° 2987894, 13/9/13 publications Stubborn contaminants influence of detergents on the purity of the multidrug ABC transporter BmrA. Wiseman B, Kilburg A., Chaptal V., Reyes-Mejia G., C., Sarwan J. Falson P.*, Jault J-M*. PLoS One. 2014 9(12) e114864. doi 10.1371. Understanding polyspecificity within the substrate-binding cavity of the human multidrug resistance p-glycoprotein. Martinez L, Arnaud O, Henin E, Chaptal V, Doshi R, Zhang Q, Chang G, Falson P. FEBS J. 2014 281 673-82 Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Ward AB, Szewczyk P, Grimard V, Lee CW, Martinez L, Doshi R, Cregger C, Swartz DJ, Falson PG, Urbatsch IL, Govaerts C, Steyaert J, Chang G. PNAS. 2013 110(33) 13386-91.
Patents
Number: 3
Detail: Method for selectively extracting membrane proteins using calixarenes for solubilization. Coleman A., Falson P., Mbemba C., Matar R. & Huché F. Brevet Français n° 0802894, déposé le 28 mai 2008. PCT/FR2009/000626 / WO 2009144419 A1 2009120 (3/12/2009). Licence CALIXAR 2010-11. Protein crystallization additives, use and process. Matar, R., Leydier, A., Huché, F., Coleman, A., Falson, P. Brevet Français n° 09/01678 déposé le 6 avril 2009. PCT/FR2010/000285 WO2010116055. Li-cence CALIXAR 2010-11. Quantification des détergents par Matrix-Assisted Laser Desorption Ionization - Mass Spectrometry (MALDI-MS) avec étalons internes deutérés et/ou structuralement proches. FALSON P., DELOLME F., RHIMI M., CHAPTAL V., PICARD M., RAVAUD, S., JAULT J-M & WAGNER, R.. 2012. Déposé le 7/3/2012 sous le n°2987894
Scientific publications
Number: 3
Detail: Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Ward AB, Szewczyk P, Grimard V, Lee CW, Martinez L, Doshi R, Caya A, Villaluz M, Pardon E, Cregger C, Swartz DJ, Falson PG, Urbatsch IL, Govaerts C, Steyaert J, Chang G. Proc Natl Acad Sci U S A. 2013 Aug 13 110(33) 13386-91 Understanding polyspecificity within the substrate-binding cavity of the human multidrug resistance p-glycoprotein. Martinez L, Arnaud O, Henin E, Tao H, Chaptal V, Doshi R, Andrieu T, Dussurgey S, Tod M, Pietro AD, Zhang Q, Chang G, Falson P. FEBS J. 2014 Feb 281(3) 673-82 Stubborn contaminants influence of detergents on the purity of the multidrug ABC transporter BmrA. Wise-man B, Kilburg A., Chaptal V., Reyes-Mejia G., C., Sarwan J. Falson P.*, Jault J-M*. PLoS One. 2014 Dec 17 9(12) e114864. doi 10.1371.
Job creation in France
Number: 12
Job creation in Auvergne-Rhône-Alpes
Number: 12
Detail: via CALIXAR, company specialized in the stabilization of membrane proteins in solution
Phd
Number: 2
Detail: Lorena Martinez PhD en 2014. à trouvé 1 emploi. Arnaud Kilburg, PhD en 2015. A repris ses études de médecine
Start-up creation
Number: 1
Detail: Calixar founded in 2011 (Lyon)
License agreements
Number: 2
Detail: with CALIXAR for the first 2 patents. 1 agreement in progress on the 3rd
Perspectives
Set-up of a new R&D project :
New ANR Project Blanc - SVSE 5 - Physique, chimie du vivant et innovations biotechnologiques 2013 "CLAMP" 2013-17 with P. Falson, IBCP LYON and Pr. A. Boumendjel, UJF Grenoble (P. Falson main investigator)