MPEC

Innovative assets

- 1 assay detergent (french patent method for qualification of detergent by mass spectrometry and its applications No. 2,987,894, 09/13/13) - 1 item in preparation of this assay, another on a membrane protein structure - 1 article: Stoichiometry of the MexA-OprM binding, as Investigated by blue native gel electrophoresis. Ferrandez Y Monlezun L, Phan G, H Benabdelhak, Benas P, N Ulryck, Falson P, Ducruix A, Picard M, Broutin I. Electrophoresis. 2012 Apr, 33 (8) : 1282-7

Highlights

1 startup créee pendant le projet (CALIXAR). 10 emplois crées. 1 brevet de dosage des détergents n°2987894, 13/9/13 publications: Stubborn contaminants: influence of detergents on the purity of the multidrug ABC transporter BmrA. Wiseman B, Kilburg A., Chaptal V., Reyes-Mejia G., C., Sarwan J. Falson P.*, Jault J-M*.  PLoS One. 2014 9(12):e114864. doi: 10.1371. Understanding polyspecificity within the substrate-binding cavity of the human multidrug resistance p-glycoprotein. Martinez L, Arnaud O, Henin E,  Chaptal V, Doshi R, Zhang Q, Chang G, Falson P. FEBS J. 2014 281 673-82 Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Ward AB, Szewczyk P, Grimard V, Lee CW, Martinez L, Doshi R, Cregger C, Swartz DJ, Falson PG, Urbatsch IL, Govaerts C, Steyaert J, Chang G. PNAS. 2013 110(33) 13386-91.

Actual results

Innovative Assets

Detail: - 1 assay detergent (french patent method for qualification of detergent by mass spectrometry and its applications No. 2,987,894, 09/13/13) - 1 item in preparation of this assay, another on a membrane protein structure - 1 article Stoichiometry of the

Highlights

Detail: 1 startup cré ee pendant le projet (CALIXAR). 10 emplois cré es. 1 brevet de dosage des dé tergents n° 2987894, 13/9/13 publications Stubborn contaminants influence of detergents on the purity of the multidrug ABC transporter BmrA. Wiseman B, Kilburg A., Chaptal V., Reyes-Mejia G., C., Sarwan J. Falson P.*, Jault J-M*. PLoS One. 2014 9(12) e114864. doi 10.1371. Understanding polyspecificity within the substrate-binding cavity of the human multidrug resistance p-glycoprotein. Martinez L, Arnaud O, Henin E, Chaptal V, Doshi R, Zhang Q, Chang G, Falson P. FEBS J. 2014 281 673-82 Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Ward AB, Szewczyk P, Grimard V, Lee CW, Martinez L, Doshi R, Cregger C, Swartz DJ, Falson PG, Urbatsch IL, Govaerts C, Steyaert J, Chang G. PNAS. 2013 110(33) 13386-91.

Patents

Number: 3

Detail: Method for selectively extracting membrane proteins using calixarenes for solubilization. Coleman A., Falson P., Mbemba C., Matar R. & Huché F. Brevet Français n° 0802894, déposé le 28 mai 2008. PCT/FR2009/000626 / WO 2009144419 A1 2009120 (3/12/2009). Licence CALIXAR 2010-11. Protein crystallization additives, use and process. Matar, R., Leydier, A., Huché, F., Coleman, A., Falson, P. Brevet Français n° 09/01678 déposé le 6 avril 2009. PCT/FR2010/000285 WO2010116055. Li-cence CALIXAR 2010-11. Quantification des détergents par Matrix-Assisted Laser Desorption Ionization - Mass Spectrometry (MALDI-MS) avec étalons internes deutérés et/ou structuralement proches. FALSON P., DELOLME F., RHIMI M., CHAPTAL V., PICARD M., RAVAUD, S., JAULT J-M & WAGNER, R.. 2012. Déposé le 7/3/2012 sous le n°2987894

Scientific publications

Number: 3

Detail: Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain. Ward AB, Szewczyk P, Grimard V, Lee CW, Martinez L, Doshi R, Caya A, Villaluz M, Pardon E, Cregger C, Swartz DJ, Falson PG, Urbatsch IL, Govaerts C, Steyaert J, Chang G. Proc Natl Acad Sci U S A. 2013 Aug 13 110(33) 13386-91 Understanding polyspecificity within the substrate-binding cavity of the human multidrug resistance p-glycoprotein. Martinez L, Arnaud O, Henin E, Tao H, Chaptal V, Doshi R, Andrieu T, Dussurgey S, Tod M, Pietro AD, Zhang Q, Chang G, Falson P. FEBS J. 2014 Feb 281(3) 673-82 Stubborn contaminants influence of detergents on the purity of the multidrug ABC transporter BmrA. Wise-man B, Kilburg A., Chaptal V., Reyes-Mejia G., C., Sarwan J. Falson P.*, Jault J-M*. PLoS One. 2014 Dec 17 9(12) e114864. doi 10.1371.

Job creation in France

Number: 12

Job creation in Auvergne-Rhône-Alpes

Number: 12

Detail: via CALIXAR, company specialized in the stabilization of membrane proteins in solution

Phd

Number: 2

Detail: Lorena Martinez PhD en 2014. à trouvé 1 emploi. Arnaud Kilburg, PhD en 2015. A repris ses études de médecine

Start-up creation

Number: 1

Detail: Calixar founded in 2011 (Lyon)

License agreements

Number: 2

Detail: with CALIXAR for the first 2 patents. 1 agreement in progress on the 3rd

Perspectives

Set-up of a new R&D project :

New ANR Project Blanc - SVSE 5 - Physique, chimie du vivant et innovations biotechnologiques 2013 "CLAMP" 2013-17 with P. Falson, IBCP LYON and Pr. A. Boumendjel, UJF Grenoble (P. Falson main investigator)